shCREATE

Sequence

Target gene Target position Minimum length Maximum length Minimum GC Maximum GC ID prefix Auto ID prefix

0 candidates

ID	Sequence	Start	End	Length	GC

BLAST output

Target gene Minimum oligo coverage Maximum mismatches Maximum E-value BLAST e-value Manual include IDs Exclusive target-gene hits only

0 selected IDs

ID	Gene hits under cutoff	Status

Forward prefix Loop Forward suffix Reverse prefix Reverse loop Reverse suffix

0 final designs

ID	Target sequence	Reverse complement	shRNA fwd	shRNA rev	Length	GC	Score